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309058

Extraction and characterization of sericin obtained from unreelable cocoons of Bombyx mori L.

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Last updated: 24 Dec 2024

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Abstract

Silkworm, Bombyx mori L. cocoon is a protective covering consisting of two important proteins, sericin and fibroin. Sericin, a water-soluble protein that represents about 30% of the cocoon weight is perse dissolved by cooking to unwind the silk filament from the cocoon during reeling. In the present study, the sericin was extracted from cut cocoons. Extraction was carried out by two methods i.e., open boiling and autoclave method at different time regimes ,30, 60 and 90 min. The recovery of crude sericin powder was done by hot air drying and freeze drying. Maximum recovery (14.12 ± 0.05 %) of crude sericin powder was obtained when extraction was carried out by autoclave method and drying by lyophilization. The sericin samples were studied for qualitative, quantitative, and biological criteria.  The autoclave extraction showed maximum protein content and protein purity as compared to open boiling method. The maximum gelation formation in the extracted samples was observed for 60 minutes followed by a time of 30 minutes. Differential molecular weight protein bands were observed at three-time intervals. A molecular weight of 15-25 KDa was obtained in the sample extracted for 30 min and 60 min. While reduced molecular weight ranges of 10-15 KDa were obtained when extraction was carried for 90 min. The sericin showed concentration dependent antioxidant activity to the tone of 64 ± 7 % at 40 mg/mL concentration. Sericin did not show any antibacterial and antifungal activity. Results of this study suggests sericin protein can be used in cosmetic, pharmaceutical and food industry. 

DOI

10.21608/ejar.2023.207010.1400

Keywords

Bombyx mori L, Sericin Extraction, SDS - PAGE electrophoresis, antibacterial and antifungal

Authors

First Name

Aabid

Last Name

Bhat

MiddleName

-

Affiliation

College of Temperate Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir (SKUAST-K), Shalimar, J&K, India

Email

aabidbhat905@gmail.com

City

Srinagar

Orcid

-

First Name

Aabid

Last Name

Tantray

MiddleName

-

Affiliation

College of Temperate Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir (SKUAST-K), Shalimar, J&K, India

Email

aabidseri@skuastkashmir.ac.in

City

srinagar,india

Orcid

-

First Name

Phalisteen

Last Name

Sultan

MiddleName

-

Affiliation

Division of Plant Biotechnology, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar - 190005, J&K, India.

Email

bioshakir@gmail.com

City

Srinagar

Orcid

-

First Name

Mir

Last Name

Maqbool

MiddleName

shahid

Affiliation

Cancer Pharmacology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar - 190005, J&K, India

Email

mirshahid070@gmail.com

City

Srinagar

Orcid

-

First Name

Sadaqat

Last Name

farooq

MiddleName

-

Affiliation

Fermentation and Microbiology Division, CSIR - Indian Institute of Integrative Medicine, Sanatnagar, Srinagar - 190005, J&K, India

Email

bhatsadaqat5@gmail.com

City

Srinagar

Orcid

-

First Name

lubna

Last Name

Altaf

MiddleName

-

Affiliation

College of Temperate Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir (SKUAST-K), Shalimar, J&K, India

Email

lubnaltaf@skuastkashmir.ac.in

City

Srinagar

Orcid

-

First Name

khursheed

Last Name

Sahaf

MiddleName

-

Affiliation

College of Temperate Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir (SKUAST-K), Shalimar, J&K, India

Email

sahafka@yahoo.com

City

Srinagar

Orcid

-

First Name

irfan

Last Name

khan

MiddleName

-

Affiliation

College of Temperate Sericulture, Mirgund, Sher-e-Kashmir University of Agricultural Science and Technology of Kashmir (SKUAST-K), Shalimar, J&K, India

Email

khan.irfan345@gmail.com

City

srinagar

Orcid

-

Volume

102

Article Issue

1

Related Issue

45266

Issue Date

2024-03-01

Receive Date

2023-04-21

Publish Date

2024-03-10

Page Start

127

Page End

138

Print ISSN

1110-6336

Online ISSN

2812-4936

Article File

EJAR_Volume 102_Issue 1_Pages 127-138.pdf

PDF . 631.2kB

Link

https://ejar.journals.ekb.eg/article_309058.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=309058

Order

12

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

Extraction and characterization of sericin obtained from unreelable cocoons of Bombyx mori L.

Details

Type

Article

Created At

24 Dec 2024