The investigation centered on the efficacy of essential oils in seedling production and the induction of resistance against globe artichoke root rot caused by Fusarium solani and Rhizoctonia solani. The study encompassed in vitro, and greenhouse experiments conducted during 2022 and 2023. The results from treatments of varying concentrations indicated that mint oil and fulvo copper yielded the most substantial inhibition, outperforming other treatments and the control group. Both light and scanning electron microscopy observations unveiled discernible alterations in the morphology of F. solani spores, showcasing a loss of plasmolysis of mycelia, and R. solani hyphae exhibited signs of degradation when treated with fulvo copper. Mint oil, clove oil, fulvo copper, and the chemical fungicide (Maxim and Rhizolex-T) were notably successful in considerably diminishing the root rot percentage and disease severity among treated artichoke seedlings compared to alternative treatments and untreated controls. Remarkably, fulvo copper demonstrated the highest enhancement in bud's number for both F. solani and R. solani cases. Moreover, treated seedlings exhibited significant growth improvements in terms of leaf count, leaf length, root and plant length, fresh weight, as well as dry matter, Crude Protein (%), Total Carbohydrate (%), Total Phenols (%), Chlorogenic Acid, and Total Chlorophyll in artichoke seedlings. Nutritional components like N (%), P (%), K (%), and Ca (%) contents showed pronounced increments upon treatment with fulvo copper. The function of defense-related enzymes, such as polyphenol oxidase, peroxidase, and catalase, demonstrated heightened levels in response to the increasing application of fulvo copper, showcasing a substantial contrast to untreated plants.