This study revealed that the best cultural conditions to produce a high yield of H.paragallinarum (HP) bacterial cells for bacterin or antigen preparation was the fermentative method. The best yield on dry weight basis was obtained by chicken meat infusion broth 'CM" with 0.5% chicken serum and brain heart infusion broth "BHI' with 1% horse serum after spreger 10% CO2 gassing in the media for four hours and incuba­tion at 37°C for 18 hours. Cultures produced by this method and diluted twice produce an effective protective bacterin and sensitive specific an­tigen. This finding has an economical value.