Eighty-four faecal samples were collected from diarrheic newborn calves. Chlamydial isolation was done in yolk sacs of embryonated chicken eggs. Impression smears werre made from yolk sac of infected chicken eggs, and stained with Geminize stain to demonstrate chlarnydial inclusion bodies. Complement fixation test (CFT) was applied on the collected sample suspension and 41 positive cases out of 84 were given at a percentage of 48.80%. Meanwhile, ELISA technique also was applied which figured in and, 46 positive cases out of 84 were figured at a percentage of 54.76%.