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326134

FAT HYDROLYSIS BY BACILLUS SUBTILIS LIPASE AND SOME PROPERTIES OF THIS ENZYME

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Last updated: 24 Dec 2024

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Abstract

The best saturation for lipase precipitation by ammonium sul­phate was found to be 0.8, which gave the highest yield, being 90.9% with specific activity and degree of purification of 70.1 p.mol FFA/mg protein/min. and 8.75 folds, respectively. By using 0.8 saturation of am­monium sulphate, pH 6.0 was found as the best pH value for enzyme precipitation. The yield of lipase at such pH value was 76.36%. The ef­fects of pH and temperature on lipase activity varied with the substrate. The optimum pH was in the range of pH 7.9; the optimum temperature was in the range 40-50°C in case of olive oil which was the best tested substrate. The pH stability curve at 4oC showed no discontinuities and had a maximum between pH 6.0 and 8.0 at 1, 24 and 48 hrs. Also at 21-50°C, the residual activity reached 88.1% and 47.6%, respectively. Moreover after 48 hrs, the residual activity showed 71.4 and 35.7%. re­spectively. The enzyme lost very little activity between 30-50°C and was also quite stable at 60°C, but it lost activity very rapidly at 80°C. The hydrolysis of oils by Bacillus subtilis lipase was also studied and the degree of hydrolysis at 40°C reached after 24 hrs 92.11, 84.62 and 79.79%, for olive, palm and coconut oils, respectively.

DOI

10.21608/ejar.1999.326134

Authors

First Name

RAMADAN A.

Last Name

HASSAN

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Affiliation

Department of Microbiology, and Agriculture Chemistry, Faculty of Agriculture, Mansoura University, Mansou‌ra, Egypt

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First Name

FATHY A.

Last Name

HAUKA

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Affiliation

Department of Microbiology, and Agriculture Chemistry, Faculty of Agriculture, Mansoura University, Mansou‌ra, Egypt

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First Name

ISMAIL I.

Last Name

ISMAIL

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Affiliation

Department of Microbiology, and Agriculture Chemistry, Faculty of Agriculture, Mansoura University, Mansou‌ra, Egypt

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First Name

TAWFIK S.M.

Last Name

SHADY

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Affiliation

Agricultural Microbiology Research Department, Soils, Water and Environment Institute, Agricultural Research Center, Giza, Egypt

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Volume

77

Article Issue

1

Related Issue

44221

Issue Date

1999-03-01

Receive Date

1997-08-06

Publish Date

1999-03-01

Page Start

15

Page End

25

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_326134.html

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https://ejar.journals.ekb.eg/service?article_code=326134

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Original Article

Type Code

1,041

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Journal

Publication Title

Egyptian Journal of Agricultural Research

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https://ejar.journals.ekb.eg/

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FAT HYDROLYSIS BY BACILLUS SUBTILIS LIPASE AND SOME PROPERTIES OF THIS ENZYME

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Article

Created At

24 Dec 2024