Bacterial strains showed maximum lipase production in Sugiura et al. (1977) basal mineral salt solution, while, Chander et a/. (1980) basal medium gave the highest amount of fungal lipase. Enzyme production was maximized in third day of bacterial growth and after five days for fungal strains. Out of carbon source tested, glucose, gave the highest enzyme yield in the culture fluids for all bacterial and fungal organisms used except of Aspergillus oryzae, sucrose was found in the first order. Maximum lipase secretion was obtained in the presence of peptone andcasin for Bacillus subtilis and Pseudomonas aeruginosa, respectively. However, wheat bran showed maximal amount of enzyme yield for fun-gal strains. Olive oil (1%) induced bacterial lipase biosynthesis, while, the addition of oil (1%) inhibited fungal lipase secretion. Addition of fatty acids to growth medium was inhibitory for lipase production.The optimal pH for 8. subtilis (12), P. aeruginosa and A. oryzae lipase production ranged from S to 7, while optimal pH for S. cerevisiae(4) and A. flavus (2) lipase production ranged from 6 to 8. The highest enzyme activity was reached at 30°C for both bacterial and fungal Strains. When, the organisms were cultivated on a shaker, the enzyme production was increased.