Twenty-four female buffaloes between 5-10 years old were used in this investigation. They were divided into two main groups according to their reproductive performance. The first group included 10 buffaloes which were normal cyclic animals and considered as control. The second group included 14 buffaloes which were anoestrum with smooth inactive ovaries. This group was further subdivided into two subgroups each of 7 buffaloes. The first subgroup was injected with vitamins AD3EC, while, the second subgroup was injected with vitamin E/Selenium. Serum vitamin A, (3-carotene, Ca, P, total protein, protein fractions, trace elements (Zn, Cu, Fe and Co), as well as progesterone hormone and cortisol were estimated. There were a significant increase in vitamin A and 13-carotene levels in both animals treated with vitamin AD3EC and vitamin E/Se. On the other hand, the levels of Ca and P were increased in treated groups with significant elevation of Ca/P ratio. Treatment with vitamin E/Se increased significantly the level of total protein more than treatment with vitamin AD3EC. The albumin was increased, while, a and 13 globulins were not significantly changed in both treated groups. At the same time,8 globulin was significantly elevated in both treated groups. The A/G ratio fluctuated within limited range without effect of supplementation. Concerning trace element levels, there was significant increase in serum Zn, Cu, and Co and Fe in both treated groups. The progesterone level was significantly increased in both treated animals, but its level was high in buffaloes treated with vitamin AD3EC than the group treated with vitamin E/Se. The cortisol level increased slightly by treatment in both groups of anoestrum buffaloes. This study cleared that vitamins and selenium supplementation play a fundamental role in fertility since injection of AD3EC and Vit. E/Selenium improved the reproductive efficiency of the anoestrum buffaloes where 85%, 71.5% of animals came in heat in both treated groups, respectively.