Three susceptible sheep were inoculated with RVF antigen mixed with incomplete and complete Freund's adjuvant and without adjuvant in three successive doses. The hyperimmune sera collected from the sheep were tested against standard reference RVF antigen for the presence of specific RVF antibodies using serum neutralization test (SNT), agar gel precipitation test (AGPT) and indirect fluorescent assay (IFA). Separa­tion of RVF immunoglobulins were done using affinity chromatography (activated sepharose 46) followed by conjugation of RVF IgG with fluo­rescein isothiocyanale at p1-19.6. The conjugatedrRVF antibodies were ti­trated against reference RVF antigen and the titre reached (1:128). The working dilution was used for evaluation of different batches of attenu­ated and inactivated RVF vaccines in comparison with IFA test using anti-sheep Fitic, the results of both assays proved to be similar.