Fifty-six does were inoculated with bovine herpesvirus-4 (BHV-4) or normal cell culture fluid by intravaginal (groups 1 & 2, early gestation) or intravenous routes (groups 3 & 4, late gestation). Forty bucks were inoculated with BHV-4 or normal cell culture fluid by intratesticular (groups 5 & 6) or intravenous (groups 7 & 8) routes. Clinical signs fol­lowing inoculation of the virus in does included abortion in 2 pregnant does, decreased conception rate (25% conception rate in group 1 vs. 41.66% conception rate in control group 2), and mild vaginitis (2124, group 1). No clinical signs were observed with viral infection in bucks. Microscopic lesions included marked placentilis, mild suppurative end*. metritis, moderate multifocal endometrial hemorrhages, endometrial oe­dema, oophoritis, mesenteric lymph node hyperplasia, and mild multifocal necrotizing hepatitis. Histopathological examination of intratesticularly inoculated rabbits revealed mild to moderate interstitial lymphocytic and acute necrotizing orchitis. with segmental to diffuse testicular degenera­tion, and mild interstitial oedema. The virus was isolated from one testi­cle of inoculated rabbit. Viral antigen was detected by immunohisto­chemistry only in the mesenteric lymph nodes and spleen in infected does, but virus was not isolated. Polymerase chain reaction (PCR) de­tected BHV-4 genome in two bucks. Transmission electron microscopy (TEM) revealed viral nucleocapsids in the uterus, vagina, spleen, and mesenteric lymph nodes of infected does and in the testis, spleen and mesenteric lymph node of infected bucks. Gross, microscopic, TEM, PCR, and immunohistochemical examination of tissues suggested that BHV-4 had a mild pathogenic role in bucks inoculated intratesticularly.