Ten barley genotypes with different responses to net blotch disease were evaluated at Sakha Agricultural Research Station during two growing seasons (2020/2021 and 2021/2022) to determine the high potential yield of the promising elite barley genotypes and resistance to net blotch disease. Earliness traits, grain yield and its attributes in addition to reaction to net blotch disease infection were studied. The analysis of variance showed significant or highly significant differences for the studied traits. Results revealed that Line 2 and Line 6 exhibited the highest values of grain yield (ton fed^-1) in both seasons under all experimental conditions. Moreover, Giza134, Line 2, Line 5 and Line 6 were the most resistant genotypes with low values for net blotch (NB) disease reaction. The correlation coefficient between the studied traits indicated that positive significant correlation between plant height with net blotch resistance (R=0.68). On the other hand, there was a significant negative correlation between the heading date and maturity date with net blotch (R=-0.73 and R=-0.67, respectively) in the second season. Concerning molecular analysis seven SSR primers including GBM1078, EBMAC0695, 5SCSSR104770, GBM1405, GBM1419, GBM1461 and GBM1516 were usedto study the polymorphic among net blotch resistance and sensitive of barley selected genotypes. All primers generated clear patterns with height polymorphism.So, genotypes (Giza 134, Line 2, Line 5 and Line 6) would be useful in breeding programs for evolving better barley yield and more resistance against (NB) disease.