The aim of the present work is to study the mechanization of the transplanting operation of cotton. A medium long Staple variety Giza 75 was transplanted using a New-Holland transplanter. U.S. make. model 1600 compared with the Egyption traditional manual method in a clay loam soil in EI-Gemmeiza agricultural research experimental station in 1998/99 season. The results may be summarized as follows : 1. The New - Holland machine, lost time was 1.53 h/fed and 1.78 h/fed in the plot leveled using laser and manually, respectively. 2. The net time needed for transplanting one feddan was 6.07 h/led and 9.33 h/fed in the plot leveled by using laser and manually respec‌tively. and increased to 76.89 h/fed for manual transplanting. 3. The field efficiency was 81.25 % and 63.33 % in the plot leveled us‌ing laser and manually, respectively. 4. Transplanting cotton consumed irrigation water less than direct seed‌ing. It was 1504 and 1880 m3/fed for plot leveled by laser and man‌ually, respectively. While, direct seeding consumed 2384 and 2980 m3/fed for plot leveled using laser and manually, respectively. 5. The growth, yield and yield components for transplanting cotton were not comparable to cotton grown by direct seeding method whether cotton was trasplanted manually or by New - Holland transplanter. The mechanical transplanting had no measurable effects on cotton trails.