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311890

A MODIFIED METHOD FOR DETECTING OOSPORES OF PERONOSCLEROSPORA SORGHI, THE CAUSAL ORGANISM OF SORGHUM DOWNY MILDEW IN SUDAN GRASS SEEDS (SORGHUM SUDANENSE)

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Last updated: 04 Jan 2025

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Abstract

The washing technique was developed for detecting oospores of the sorghum downy mildew (Peronoscierospora sorghn on seed surface or in fine plant debris (seed transmitted infection) in sudan grass (Sor­ghum sudanense). Modification was based on grinding the seed bulk for 2 min before washing followed by I ifferation,centrifugation and adding drops of iodine tincture to the washing sediments. Grinding increased the number of oospores in washing sediment from the different seed parts , i.e., glumes , pericarp or perianth as well as from plant debris. On the other hand , iodine tincture stained the starch particles, prolonged the time provided for microscopic examination and made the examination easier than in the common technique. Although mycelium was observed also in some different seed parts, especially the endosperm tissues, the internal infection (endosperm and scutellum) could not be confirmed yet. Systemic infection of plants produced from infested seeds was proved in the greenhouse using sterilized soil (pathogen-free). Mean­while, no systemic infection was observed in plants resulting from non-infested seeds. Moreover, soaking sudan grass seeds in a suspension of the fungicide Apron (metalaxyl) at Concentration of 750 ppm for 2 hours before sowing ,increased the emergence and reduced seed trans­mission of Peronosclerospora sorghi.

DOI

10.21608/ejar.2002.311890

Authors

First Name

MOHAMED A.

Last Name

SHAARAWY

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Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

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First Name

ABD-ALAAH M.

Last Name

ABDEL-MONEM

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Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

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First Name

ABDEL-FATAH A.

Last Name

EL-WAKIL

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Affiliation

Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

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Volume

80

Article Issue

3

Related Issue

42781

Issue Date

2002-09-01

Receive Date

2001-01-20

Publish Date

2002-09-01

Page Start

1,033

Page End

1,044

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_311890.html

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https://ejar.journals.ekb.eg/service?article_code=311890

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5

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Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

A MODIFIED METHOD FOR DETECTING OOSPORES OF PERONOSCLEROSPORA SORGHI, THE CAUSAL ORGANISM OF SORGHUM DOWNY MILDEW IN SUDAN GRASS SEEDS (SORGHUM SUDANENSE)

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Article

Created At

24 Dec 2024