A mycorrhizal fungus Glomus aggregatum (VAM), and a bacterial isolate Bacillus subtilis (Bs), were evaluated individually or in combination with Bradythizobium japonicum (Rh) as biocontrol agents for reducing root-rot disease caused by fusarium oxysporum (P.O.) in soybean plants, under greenhouse conditions. In non-inoculated (control) plants, infesta­tion of soils with F.O. drastically reduced plant growth parameters com­pared to non-infested ones. However, the detrimental effects caused by F.O. infestation were less detected in VAM and/or Bs inoculated treat­ments, being the least pronounced in the VAM+Bs treatment. In plants inoculated with B. japonicum, fusarium infestation result­ed in variable decreases in number and dry weight of nodules, plant growth and seed yield. However, rhizobial inoculated-plants could with­stand the stress of fusarium infestation when biologically controlled with G. aggtegatum and/or B. subtilis. Results pointed out that the observed prophylactic effects of mycorrhizal inoculation was not only related with plant nutrition, but also related with reduction of disease severity.