One of the most significant banana diseases in Egypt is Banana Bunchy Top Disease, which is brought on by the Banana Bunchy Top Virus (BBTV). The creation of BBTV-free plantain seedlings from infected banana plants was the main goal of this investigation. Four separate governorates provided samples (Qalubyia, Giza, Ismailia, and Behira). All collected samples underwent BBTV testing using DAS-ELISA, which revealed that the virus infection was recorded in all inspected governorates in different ratios. The diagnostic host of BBTV was limited, infecting banana, ginger, and bird of paradise using mechanical inoculation, while only banana and ginger were infected using insect inoculation with P. nigronervosa. Polymerase chain reaction (PCR)-based detection of an amplicon from BBTV-infected banana tissues (476 bp) The multiple sequence alignment showed that EGY-Behira isolates had a high similarity of 99.7% with isolate BBTV-replicate from India. The phylogenetic tree for the BBTV-Eg Behira isolates is based on the partial nucleotide sequence alignment of the DNA-A genome. BBTV was successfully eradicated from infected plants using a combination of meristem tip culture and thermotherapy at 40 ^oC for two months. Virazol and Salicylic acid provided the greatest polyphenol oxidase activity (1.64 and 1.78) as well as highly enzymatic activity (2.021 and 1.96 for peroxidase). Significant biochemical changes were represented by a relative reduction in Chl a, Chl b, and carotenoid contents. It was observed that the highest reduction in chlorophyll b was 51.47%, followed by chlorophyll a+b at 51.27, chlorophyll a at 51.17, and carotenoids at -2.65, respectively.