Bio-oils are a complex material that is produced through the condensation of vapors raised from the pyrolysis of plant biomass components and consist mainly of phenolic compounds, benzene derivatives, and organic acids. Few studies have been conducted on the antifeedant efficiency of bio-oils. This study aims to evaluate the chemical composition, toxicity, and antifeedant activity of bio-oil produced from the pruning residue of Cupressus sempervirens in addition to two types of essential oils, Myrrh and Frankincense oil, against Sitophilus oryzae. The GC/MS analysis of the essential oils showed Diethyl Phthalate (75.15%) in myrrh oil, alpha-Thujene (30.70%), and Octyl acetate (20.44%) in frankincense oil. The results clearly showed the high toxicity of essential oils, myrrh and frankincense, and the low toxicity of bio-oil against S. oryzae after 7 days of exposure time, while the while the combined use of myrrh oil and frankincense oil with bio-oil increased the toxicity against S. oryzae about 1.91 and 2.27-fold, compared to myrrh oil and frankincense oil alone. In all treatments, the antifeedant parameters revealed that myrrh oil had higher nutritional indices than Frankincense oil. C. sempervirens showed a significantly low antifeedant efficacy compared to both essential oils. Generally, the values of relative growth rate (RGR), relative consumption rate (RCR), and efficiency of conversion of ingested food, (ECI) significantly decreased, and, contrariwise, the FDI increased in all treatments compared to the control.