The present work was conducted for two successive seasons, i.e. 1996 /1997 and 1997 /1998 in Shandaweel Research Station. Agricultural Research Center, Souhag Governorate. The aime of this study is to invistigate the effect of fertlization on yield and yield components of some sugar cane varieties.This experiment included 18 treatments which were the combination between two sugar cane varieties (G.T.54-9 and G.85-37) and the following nine fertilization treatments: (180 kg N /fed.,120 kg N / fed., 60 kg N / fed., inoculation with Azotobacter +60 kg N /fed., inoculation with Azotobacter + 120 kg / fed., inoculation with Azospirillum + 60 kg/fed., inoculation with Azospirll/umm +120 kg / fed., inoculation with azotobacter only and inoculation with Azospirillum only). "- Sugar cane variety G.85-37 surpassed G.T.54-9 variety in respect to net cane yield . G.85-37 attained a distinct increment amounted to 19.18% and 6.5% over those of the commercial variety in the 1st and 2nd season, respectively. *-There was a pronounced superiority in the values of net cane yield for the combination between any of the examined biofertilizer (Azotobacter and/or Azospirillum) with the middle dose of the mineral nitrogen 120 kg N over those resulted from biofertilizer or mineral nitrogen fertilizer alone, in the ist season. *- Sugar cane variety G.T.54-9 recorded the highest value of sugar recovery(S.R. %) in the lst season (12.87 %). However,the difference between the two varieties in that respect was insignificant in the 2nd season. *- The highest sugar recovery value (12.933%) was recorded by applying 180 kg N/fed. Similar result was obtained when sugar cane seed setts were inoculated by Azospirillum alone. *- The promissing sugar cane variety 0.85-37 significantly surpassed the commercial one in respect to sugar yield, in both seasons. The relative advantage in sugar yield for G.85-37 variety over that of G.T.54-9 variety amounted to be 13.843% and 5.658% in 1sI and 2" season, respectively. *- Sugar yield was statistically affected by fertilization treatments. The highest values of sugar yield were gained when sugar cane seed setts were inoculated by Azotobacter and/or Azospirillum in addition to 120 kg N/fed