The present work was conducted for two successive seasons, i.e. 1996 /1997 and 1997 /1998 at Shandaweel Research Station, Agricul­tural Research Centre, Sohag Governorate. The aim of the study is to in­vestigate the effect of bicfertlizer on growth characters, chemical com­ponents, juice quality, yield and yield components of sugar cane varieties.The experiments included 18 treatments which were the combi­nation between two sugar cane varieties (G.T.54-9 and G.85-37) and nine fertilization treatments: 180 kg N /fed.,120 kg N / fed., 60 kg N / fed., inoculation with Azotobacter +60 kg N /fed., or +120 kg / fed., in­oculation with Azospirillum +60 kg/fed., inoculation with Azospirillumm +120 kg / fed., inoculation with Azotobacter only and inoculation with Azospirillum only. Azotobacter chrooccocum and Azospirillum brasilense were ob­tained from soil, water and environmental research institute, agricultural research center Giza. Exprimental treatments were arranged in complete randomized block design with four replications. A combined analysis for the two sea­son was done. The important results could be summarized in the follow­ing: "-Sugar cane varieties had no significant effect on N % ,Na % , Brix and sucrose % , however, K % , purity % ,and sugar recovery were sig­nificantly affected by the examined varieties. The lower value of fiber % was recorded with the commercial variety "G.T.54-9". "-Application of biofertilizer sources i.e Azotobactor and Azospritlum with 120 kg.N/fed. improved N % and recorded the lowest value of K % and appropriate of fiber % . The highest value of sugar recovery (12.33 % and 12.23)was recorded by applying 180 kg.N /fed, inculat­ed seed setts by Azotobacter chrooccocum + 60 kg.N /fed respec­tively, however, effect of nitrogen sources on Na % , K % , Brix %, Re­ducing sugar % , Sucrose % and purity was insignificant.