Ten Ralstonia solanaceannn isolatos were obtained from Spunta and Nicola potato cultivars . PCR-based RAPD analysis was carried out, using four primers, on agarose and polyacrylamide gels as support media. Only, the primer 6-d (AACGCGCAAC)-3 differentiated the Isolates recov­ered from the two cultivars, others did not. In general, separation on poly-acrylamide and staining with silver nitrate gave better resolution than those on agarose and stained with ethidium bromide. Further inves­tigations are needed using greater number of :solates, from different po­tato cultivars and /or different sources, to confirm the result as well as the possible effect of the host cultivars on the properties of bacterial DNA.