Background: The workers in butane gas tubes packing plant factory are usually exposed to various occupational health hazards due to the nature of work so, they need support to overcome this hazards. Aim: to assess knowledge and safety practice of workers about occupational hazards in butane gas tubes packing plant factory. Design: Descriptive design was carried out. Setting: In the factory of butane gas tubes packing plant factory in Akhmim city at Sohag Governorate. Sample: (Total coverage) all numbers  of the workers was chosen to carry out the study. Tools: Two tools were used for data collection. Tool (I) divided into four parts; part (1): personal data of the subject, part (2): medical history, part (3): the workers exposure in workplace, Part (4): Knowledge of the workers about the occupational health hazards and preventive measures. Tool (II) An observational checklist that included preventive measure & safety practice. Results: 62.8% of the studied subjects were exposed to health hazards, 53% of them had a poor knowledge about the health hazards, 62.2% of them incompetent with the safety practice and preventive measures. Conclusion: The workers had a poor knowledge about the occupational health hazards. Also, they had unsatisfactory report about safety practice and preventive measures. So, most of them were exposed to the occupational health hazards. Recommendation: Training and educational program about the preventive measure in the factory to eliminate the hazards in workplace.