Background: Staff nurses play a vital role in delivering continuous, safe, high-quality and effective healthcare services, which are in fact hampered by unfavorable interpersonal experiences like ostracism and the associated alienation at work. Aim: To examine the workplace ostracism and its relationship with job performance and work alienation among staff nurses. Research design: A descriptive, correlational research design was utilized. Setting: This study was conducted at Itay El-Baroud General Hospital. Subjects: 303randomly selected staff nurses. Tools: Three tools were used: Tool I: Workplace Ostracism Questionnaire; Tool II: Observed Nursing Job Performance Scale; and Tool III: Work Alienation Questionnaire. Results: Current study illustrated that 38.6% of staff nurses had moderate level of workplace ostracism; 77.2% of them had low level of job performance; and more than half of them (55.4%) had moderate level of work alienation. Conclusion: There was a statistically significant negative correlation between total workplace ostracism and job performance among staff nurses; and there was a highly statistically significant positive correlation between total workplace ostracism and work alienation. Recommendations: Healthcare organizations should promote staff nurses' participation in workshops focused on fostering healthy interpersonal relationships and strengthening mutual respect, clear expectations and trust among staff nurses and their colleagues.