Background: Performance reviews have an impact on worker motivation, interactions with managers, and when workers believe reviews are fair, they encourage cooperative conduct. Aim: The study aimed at investigating the effect of nurse managers training program about performance appraisal on staff nurses' perception of its fairness and collaboration behavior. Design: Quasi-experimental strategy was implemented. Setting: The Oncology Institute, Minia University Kidney and Urology Hospital and Minia University Liver Hospital, Minia governorate, Egypt. Sample: Convenient sample of available on-duty head nurses (n = 41) and staff nurses (n = 324) worked in the afforded hospitals at data collection time. Tools: Three tools were used: 1- Head Nurses Knowledge of Performance Appraisal Questionnaire, 2-Perceived Fairness of Performance Appraisal Scale, and 3-Nurse-Nurse Collaboration Scale. Results: Through post training-measures (post & follow-up tests) 90.2% head nurses are well-informed about performance appraisal, in addition, 76 % and 87.3% staff nurses reported high levels of perception regard performance appraisal fairness as well collaboration behavior in arrow after the training program implementation. Conclusion: Head nurses' knowledge level increased significantly between the pre/post and follow up program periods. In addition, marked improvement monitored in nurses' perception of performance appraisal fairness as well as collaboration behavior in post and follow up program phases than before. Recommendations: Evaluate staff nurses' opinions on teamwork and fairness of appraisals on a regular basis. Nurse supervisors should regularly take refresher courses to stay current on best practices in performance evaluations.