Background: Lean Six Sigma (LSS) is a tool designed to address issues and enhance the efficiency and effectiveness of healthcare agencies by reducing variation and eliminating waste. To ensure the sustainable implementation of LSS, hospitals must be adequately ready to adopt this approach. Aim: assess health care organizations for lean six sigma implementation. Design: in this study, a descriptive research design was adopted. Setting: study conducted at a private hospital in Cairo. Subjects: a convenient sample of nurses (158) working in the forementioned study setting. Data collection: there were two tools used as, 1st tool. nurses' knowledge questionnaire and 2nd tool: Healthcare readiness questionnaire toward lean six sigma implementation. Results: less than half of nurses have a low level of knowledge while 23.4% have a high level of knowledge regarding lean six sigma. More than half of nurses perceived a low level of readiness while 22% perceived a high level of readiness. Conclusion: there was a statistically positive significant correlation between knowledge and healthcare organizational readiness at r= 0.45 p=< 0.0001. Recommendations: establish ongoing training and education sessions focused on Lean Six Sigma principles, methodologies, and practical applications. Further studies to evaluate the effectiveness of various training and educational interventions designed to improve Lean Six Sigma knowledge and readiness among healthcare staff.