Background: Workplace spirituality is one of the most important issues that improves organizational sustainability and nurses' job fit. Aim: To explore the influence of workplace spirituality on organizational sustainability and person-job fit among nurses at Gastroenterology Surgical Center and Health Insurance Hospital. Design: Descriptive correlational comparative design. Setting: The study was carried out at Gastroenterology Surgical Center and Health Insurance Hospital at Mansoura City. Subject: All nurses working in Gastroenterology Surgical Center (120) and Health Insurance Hospital (175) who meet the criteria of having a minimum of one year of experience were included in the study. Tools: Three tools were used; Workplace Spirituality Scale, Organizational Sustainability Scale, and Person-Job Fit Scale. Results: A statistically significant positive correlation was observed between workplace spirituality and organizational sustainability at both study hospitals. Dimensions of workplace spirituality had significant relationship with dimensions of organizational sustainability. Also, a statistically significant positive correlation was found between workplace spirituality and person-job fit in both hospitals. There were statistically significant relations among all items of workplace spirituality and all items of person-job fit at both study hospitals. In addition to that, Gastroenterology Surgical Center has the highest mean score of total workplace spirituality, total organizational sustainability and total person-job fit in comparison to Health Insurance Hospital. Conclusion: Workplace spirituality has a significant influence on increasing organizational sustainability and person-job fit. Recommendations: Enhancing organizational sustainability through effective utilization of human resources practices including strategic human resource planning, selective hiring and in-depth training.