Background: One of the most crucial approaches to preventing genetic diseases, congenital defects, and a number of medical and psychosocial marriage issues is premarital care. It may present a chance to act in response to the risk that has been discovered. Aim: Assess knowledge and Attitudes of nursing students of secondary school Technical girls' school towards premarital care. Sample: Involved 190 students, they used as a convenient sample. Research design: Descriptive cross sectional research design. Setting: The study conducted at Assuit Nursing Secondary School Female Students at Al-Eman General Hospital in Assuit city. Tools: Two tools were used, self-administered questionnaire that involved two parts, and attitude scale regarding premarital care. Results: About 71.6%, 26.3%, and 2.1% of the studied nursing students had poor, average and good knowledge respectively, 92.1% and 7.9% of the studied nursing students had positive and negative attitude respectively, a highly statistical significant relationship between the studied nursing students' total knowledge and attitude regarding premarital care at p-value< 0.01. Conclusions: Most of the studied nursing students had a poor knowledge and the majority of them had positive attitude regarding premarital care. Recommendation: arranging social worker-led counseling sessions in schools to educate students about premarital care.