Background: Recent reports of multiple Human Monkey Pox (HMPX) cases from several non-endemic nations in Europe, North America, and Oceania raise the possibility of an unexpected and concerning public health problem. Aim of the research: To examine the effect of educational intervention on knowledge and attitudes regarding Human Monkey Pox among primary school teachers in rural areas at Minia district. Research design: A quasiexperimental approach was utilized. Setting: The research was conducted at 5 governmental primary schools in rural areas at Minia district-Minia Governorate in Upper Egypt. Sample: A systematic random sampling of 180 primary school teachers. Tools of data collection: A self-administered questionnaire included the following parts: Part I: socio-demographic characteristic. Part II: participants' knowledge. Part III: participants' attitude. Result: About 18.3% of the studied teachers have good knowledge about human monkey pox pre teaching program compared with 71.1% of them have a very good knowledge post-teaching program. There are statistical significant differences in the subjects' level of knowledge and attitude regarding Human Monkey Pox after implementation of the educational intervention while the p value is 0.0001, 0.0001 respectively. Conclusion: Implementation of educational intervention was effective in raising the studied subjects' awareness regarding Human Monkey Pox. Recommendation: Continuous implementation of educational interventions regarding Human Monkey Pox among school population to enhance public awareness, raise the precautionary behaviors as well as mitigate the potential crisis.