Background: Cystoscopy is one of the most frequent urological procedures. Aim: To evaluate the effect of applying nursing instructions brochure on the outcomes of patients undergoing cystoscopy. Research design: Quasiexperimental research design. Setting: The study was conducted at Assiut University Urology Hospital (male, female urology departments and outpatients). Sample: One hundred patients were divided into two equal groups; study and control groups. Tools: (I): Patient assessment form. Part (I): Socio-demographic data for the Patient, Part (II): Medical data, Part (III): Patient knowledge assessment form. Tool II: Beck anxiety inventory. Tool (III): Patient satisfaction questionnaire. Results: the majority of both groups had never a cystoscopy. Prostate enlargement affected more than 25% of the study group, while hematuria affected 32% of the control group. Conclusion: Significant improvement in knowledge, anxiety, and satisfaction level for study group after applying nursing instructions brochure. Recommendation: Nursing instructions brochure for patients undergoing cystoscopy should be available for patients undergoing cystoscopy.