Background: Postpartum period is a crucial time for the body to adjust, and the mother goes through a lot of physical and mental struggles during this time, which can have an intensive impact on the health of the mother and newborn in the future. The aim: This study aimed to assess the knowledge about postpartum care among women at Women's Health Hospital, Assiut University. Research design: A Descriptive, cross sectional design was used to achieve the objective of this study. Setting: This study was conducted at emergency unit & postpartum department at Women's Health Hospital, Assiut University. Sample: convenient sample on 300 post-partum women. Data collection: Data were collected by structured interview questionnaire consisted of three parts: Part 1: Included sociodemographic data, medical history and obstetric history Part 2: knowledge about maternal care, Part 3: knowledge about neonatal care. Results: The current study findings showed that only 8% of the studied postpartum women had good while 59.7% had poor knowledge regarding postpartum care. There were a high significant relation between total score of women's knowledge and their residence, educational level and employment status. Conclusion: About more than half of the postpartum women had poor knowledge regarding postpartum care. Recommendation: give health education supported by drawing pamphlets about postpartum self and neonatal care on discharge for postpartum the women