Background: Advances in cancer treatment have a significant impact on the survival rate of gynecologic female cancer patients. Oocyte cryopreservation is one method females can use to keep their fertility. Aim: Evaluate the effect of educational package regarding oocyte cryopreservation on knowledge and attitude of gynecological cancer females. Design: Quasi- experimental design of one group was utilized. Setting: At Al-Shifa Medical Complex and Radiotherapy Center at Al Sulaiman Private Hospital in Port Said. Sample: According to the inclusion criteria, 374 female cancer patients were included in the purposive sampling. Tools: Two tools were used includes structured interview and attitude Likert scale regarding oocyte cryopreservation. Results: Before intervention, Mean and SD of total score of knowledge and attitude were poor (.82±.523 & .48±.862), while at the post-intervention; there was an improved to 1.80±.535 and 1.80±.606. Also at follow up there was an improvement to 1.70±.647 and 1.64±.776 compared to pre intervention phase with statistically significant (P=0.000*). Also, there was a significant, positive correlation between knowledge and attitude throughout the program phases (P=0.000*). Conclusion: The educational package was highly effective at changing the female cancer patients' knowledge and attitudes regarding fertility preservation as an innovative approach to maintain their future ability for reproduction. Recommendations: The importance of including this innovative approach as one of the foundational nursing curricula for female patients who have just received a cancer diagnosis, as well as evolving protocols and pathways to empower prompt health education and communication between the healthcare team and newly diagnosed female cancer patients regarding fertility preservation options.