Children receiving hemodialysis suffer from several psychological complications, and they need to receive safe, feasible, and applicable interventions to enhance their psychological wellbeing. Aim of the study was to evaluate the effect of intradialytic physical exercise on stress level among hemodialysis children. A quasi experimental research design was utilized in this study. Subjects and Method were children on hemodialysis from 6 to 18 years' old, enrolled at pediatric hemodialysis units of Assiut University Children's Hospital. They were selected and allocated randomly into two groups: study group who received intradialytic physical exercise and control group who received routine hospital care. Two tools were used to gather the required data, which included structured interview questionnaire for personal and medical data and perceived stress level scale. Results: After two months of intradialytic physical exercise, it was displayed that the majority of children in the study group had a low level of stress compared to children in the control group with highly statistically significant difference between the studied children according to total perceived stress level. Conclusion: Intradialytic physical exercise significantly decrease stress level among hemodialysis children. Recommendation: Intradialytic physical exercise should become an essential component of routine nursing clinical practice for hemodialysis children to improve psychological stress.