Background: Organizational cynicism exist as a resistance against improvement of the organization as it has adverse effects such as distrust, burnout, alienation and decrease organizational loyalty. Aim: Assess organizational cynicism and work alienation among nurses and its relation to organizational loyalty. Research Design: A descriptive correlational design was used. Setting: The study was conducted in all inpatients units of medical and surgical departments at Benha University Hospital. Sample: Convenient sample of nurses (317) out of 1528. Tools of data collection: Three main tools namely; (I): organizational cynicism scale, (II) work alienation scale and (III): organizational loyalty questionnaire Results: More than two fifth (43.8%) of nurses had high level of organizational cynicism and more than half (57.4 %) of them had moderate level of work alienation. While, more than two fifth (44.8 %) of nurses had moderate level of organizational loyalty. Conclusion: There was a statistical significant positive correlation between total organizational cynicism and total work alienation. On the other hand, there was a statistical significant negative correlation between total organizational cynicism and total organizational loyalty. Also, there was a highly statistical significant negative correlation between total work alienation and total organizational loyalty. Recommendations: Providing the autonomy and support for nurses by building an atmosphere of confidence, trust, respect and justice to minimize cynicism and work alienation and evaluating the effect of organizational cynicism and alienation on nurses' productivity.