Background: Female sexual dysfunction is a fairly prevalent and generally disregarded problem in the general population. It is a multi-factorial phenomenon. Different personal, medical, obstetrical and environmental factors affect sexual function. Aims: Assess the prevalence and risk factors of Female sexual dysfunction and to identify the barriers perceived by women to initiate discussion of sexual dysfunction issues. Design: cross-sectional study design. Subjects: A stratified random sample consisted of (600) women at different work places in the Assuit university faculties such as (faculty of nursing, Arts, Medicine, Pharmacy, science, etc.,…), the whole number of women in these faculties are very large so, we was chose a stratified random sample about 5 %of each faculty. Tools: A structured questionnaire consist of two tools (female history, FSFI) Results: Over two thirds of the women in the study had female sexual dysfunction (69.0%). The most risk factors are age, hypertension, DM, FGM, vaginal delivery and operative vaginal delivery. Majority of women (87.83%) felt shame in discussing their sexual problem Conclusion: Female sexual dysfunction is extremely common in Assuit university faculties. And there are some risk factors for it as age, DM, hypertension, vaginal delivery. Shame was the first barrier perceived by women in discussing this problem with Health Care providers. Recommendations: Special attention is needed by the health care providers for pre-marital counseling about sexual health.