Background: Respiratory syncytial virus (RSV) is the causative agent of both pneumonia and bronchiolitis during childhood presenting with varied signs and symptoms vary from mild upper respiratory tract infections to severe fatal lower respiratory tract infections which require hospitalization. Aim of the study: was to evaluate the effect of self-learning package on nurses' performance regarding care of children suffering from respiratory syncytial virus. Study design: A quasi- experimental approach was used in the present study. Sample: A convenience sampling of nurses (n=59) and children (n=59) at Pediatric medical department and Pediatric Intensive Care Units at Benha University Hospital. Tools of data collection: four tools were used namely; a structured interview questionnaire form, Observational Checklists, Nurses' attitude in relation to caring of children suffering from RSV and Children medical assessment form. Result: There was a highly statistically significant difference between the pre and the post of self-learning package regarding to nurses' knowledge, practice and attitude in relation to caring children suffering from respiratory syncytial virus (p < 0.000). Conclusion: Implementation of self-learning package improved the studied nurses 'knowledge and enhanced their practice and attitude in relation to caring of children suffering from RSV. Recommendation: Similar studies are encouraged to be done on larger sample of children from different age groups and regions for generalization of the results.