Background: Ureteroscopy is an important diagnostic and therapeutic procedure. Nursing education is an essential part in reducing postoperative complications and improve recovery following ureteroscopy. Aim: Evaluate the effect of nursing instructions on quality of recovery for patients undergoing ureteroscopy. Methods: A quasi-experimental design was used for a sample of 120 male and female adult patients underwent ureteroscopy at Urology and Nephrology Assiut University Hospital. Patients divided on random basis equally into study and control groups (60 patients for each). Nursing instructions following ureteroscopy (brochure) were introduced to patients of study group while patients of control group allocated to routine hospital nursing instructions. Patients were followed up for 3 weeks. Tools: Assessment questionnaire for patient undergoing ureteroscopy, EuroQol-5 dimension, visual analogue scale of the EQ-5D-5L, the 28-item of the La Monica–Oberst patient satisfaction scale. Results: A statistically significant improvement regarding pain level and health-related quality of life were found among the study group than among the control group with statistically significant different with (p < 0.001). Also, the incidence of postoperative urinary tract infection in the study group was significantly lower than among the control group with (p <0.01). Conclusion: Nursing instructions significantly showed positive effect on improving pain level and quality of life, lowering incidence of urinary tract infection, and increasing satisfaction level among study group patients. Recommendation: Brochure for patients about ureteroscopy nursing instructions should be available in the urology hospitals.