Background: Teamwork and organizational agility are crucial components for any organization, particularly healthcare organizations, to achieve the necessary level of performance and desired outcome. Aim: To examine organizational agility and its relation with nursing manpower teamwork attitude at Beni-Suef University Hospital. Methods: A descriptive correlational design was utilized with a sample of (290) nursing staff at Beni-Suef University hospitals. Tools of data collection were organizational agility survey and nursing manpower teamwork attitude questionnaire. Results: Two thirds (61.7%) of studied sample had moderate level of perception of organizational agility and majority (80.3%) of studied sample had good perception toward teamwork. There was a statistically significant positive relationship found between organizational agility and teamwork. Conclusion: There was a statistically significant positive correlation between organizational agility and teamwork at Beni-Suef University Hospital. Recommendations: Create training programs on how to adapt to change, how to deal with resistance to change, and how to increase agility and flexibility in a dynamic environment for all staff members. Enhance teamwork culture by developing of workshops, programs for ongoing education, and seminars in order to strengthen interpersonal relationships and increase employee engagement among nursing staff.