The aim of the current study was to evaluate the possible protective influences of Nano selenium (Nano Se), probiotics and vitamin C against ochratoxin-A (OTA) induced hepatorenal injury and oxidative stress in male rats. Forty-five albino rats were divided into 9 equal groups. G1:  Control, G2: Ochra 50 µg/kg b.wt, G3: Ochra + Nano Se 0.31 mg/kg b.wt, G4:  Ochra+ vitamin C 1000 mg /L water, G5:  Ochra+ Probiotic 400 g/ton of diet, G6:  Ochra + Nano Se + vitamin C, G7:  Ochra + Nano Se + Probiotic, G8:  Ochra + Nano Se + vitamin C+ Probiotic, G9: Ochra + vitamin C + Probiotic. The obtained results showed significant increase in serum AST, GGT, ALT, and LDH activities, Creatinine, urea, TNF-α, IL-6, SOD, CAT, MDA, alpha 1, gamma 2, T. alpha and T4 concentrations in OTA intoxicated rats. Meanwhile, a significant decrease in GSH, TAC, total protein and its fractions including (albumin, Total beta, Total gamma, Total globulin, alpha 2, beta 1, gamma 1), albumin/globulin (A/G) ratio in addition to serum testosterone, estradiol, TSH and T3 concentrations were observed in OTA exposed rats. The results suggested that NanoSe, probiotics, and vitamin C are strong antioxidants agents that might ameliorate OTA-induced renal and liver damage in rats by combating free radical and attenuate oxidative stress. Also, Nanoselenium, probiotics, and vitamin C, were found to be effective in protecting rats against OTA-induced hepato-renal injury by anti-inflammatory mechanism with the best result of Nano selenium or probiotic alone and their combination.