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287171

Sea cucumber (Holothuria atra) ethyl-acetate extract exerts anti-cholinesterase properties

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary pharmacology

Abstract

Alzheimer's disease (AD) is characterized by hyperactivations of acetylcholinesterase (AChE) associated with neuroinflammation. Sea cucumber has anti‑inflammatory, immunostimulant, and anticancer properties. It is considered a good nutraceutical candidate with low toxicity and high efficiency. This study assessed the activity of well-characterized sea cucumber- ethyl acetate crude extract toward AChE in vitro. Sea cucumber (Holothuria atra) samples  collected from Hurghada were extracted by the maceration method using ethyl-acetate (99%), making a 0.12% yield. Furthermore, the extract phytochemical ingredients were identified using GC-MS. AChE enzyme was purified from male Egyptian Mediterranean bull brain tissue using ammonium sulfate precipitation and gel filtration (Sephadex G-100). The purification profile was assessed by polyacrylamide gel electrophoresis. The inhibitory effect of the H. atra crude extract toward AChE was assessed colorimetric, and the kinetics profile was studied to identify the inhibition type by calculating Km and Vmax. Finally, in vitro cytotoxicity profile of this extract toward WI38 cells was estimated. The results revealed that the AChE was properly purified as the purification fold was increased by 27 times. Ethyl acetate crude extract demonstrated an uncompetitive AChE inhibition profile with an IC50 value of 59.07 µg/ml toward WI38 cells. The results of the present investigation support the hypothesis that the crude ethyl-acetate extract of sea cucumber has anti-AChE properties.

DOI

10.21608/djvs.2022.156975.1089

Keywords

Anti-Alzheimer's, Acetylcholinesterase inhibitors, lolly fish, Cytotoxicity, GC-MS

Authors

First Name

Marwa

Last Name

Zayed

MiddleName

Ashraf

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

marwa.zayed3000@gmail.com

City

Alexandria

Orcid

29508271800781

First Name

Mohammed

Last Name

Abd El Hafez

MiddleName

Said

Affiliation

National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt, Center of Excellence for Drug Preclinical Studies (CE‑DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt

Email

mohamedsaid80@yahoo.com

City

Alexandria

Orcid

0000-0002-7223-2340

First Name

Miral

Last Name

Abd Elwahab

MiddleName

Gamal

Affiliation

Center of Excellence for Drug Preclinical Studies (CE‑DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt

Email

dr.miral2013@yahoo.com

City

Alexandria

Orcid

0000-0002-4108-7136

First Name

Rehab

Last Name

Mady

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

rehabmady1976@gmail.com

City

Damanhour

Orcid

-

First Name

Gaber

Last Name

Batiha

MiddleName

El-Saber

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

gaberbatiha@gmail.com

City

Damanhour

Orcid

0000-0002-7817-425X

First Name

Hazem

Last Name

Shaheen

MiddleName

M

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Email

dr_hazemshaheen3010@gmail.com

City

Damanhour

Orcid

0000-0001-5931-0466

First Name

Doaa

Last Name

Ghareeb

MiddleName

Ahmed

Affiliation

Center of Excellence for Drug Preclinical Studies (CE‑DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt, Bio‑screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt

Email

d.ghareeb@alexu.edu.eg

City

Alexandria

Orcid

0000-0003-3327-4377

Volume

9

Article Issue

1

Related Issue

38024

Issue Date

2022-12-01

Receive Date

2022-08-18

Publish Date

2023-02-23

Page Start

29

Page End

35

Print ISSN

2636-3003

Online ISSN

2636-3011

Article File

DJVS_Volume 9_Issue 1_Pages 29-35.pdf

PDF . 352.8kB

Link

https://djvs.journals.ekb.eg/article_287171.html

Detail API

https://djvs.journals.ekb.eg/service?article_code=287171

Order

287,171

Type

Original Article

Type Code

1,001

Publication Type

Journal

Publication Title

Damanhour Journal of Veterinary Sciences

Publication Link

https://djvs.journals.ekb.eg/

MainTitle

Sea cucumber (Holothuria atra) ethyl-acetate extract exerts anti-cholinesterase properties

Details

Type

Article

Created At

24 Dec 2024