Enterotoxaemia (pulpy kidney) is a severe condition that has a very brief clinical course in small ruminants (young sheep and goats) and results in unexpected deaths. Therefore, therapeutic interventions often do not work. It is brought on by the absorption of the Clostridium perfringens (C. perfringens) type D toxin from the intestinal tract. This bacterium has five toxin types, and type D, which produces epsilon toxin, is primarily linked to enterotoxaemia in caprine and ovine species. This study aimed to improve the efficacy of the immunization by investigating the toxigenicity of C. perfringens type D and the impact of each element of its production media on the potency of the epsilon toxin, three types of sugars (glucose, sucrose and Dextrin) were tested with four types of proteins (peptone, tryptone, protease peptone and N-Z casein) with different incubation periods (18, 24 and 48 hours) to improve the media to reach to maximum toxigenicity. Among all these trials, sucrose with N-Z casein with 24 hours' incubation has been ascended the throne.