The occurrence of hypophosphatemia in Egyptian buffaloes is acknowledged as a significant metabolic disorder with a high frequency. This research focuses on highlighting certain clinical and biochemical alterations in buffaloes experiencing subclinical hypophosphatemia(SCH). A clinical examination was conducted on 35 affected buffaloes and an additional 10 buffaloes that appeared to be healthy. The results of clinical examination revealed that SCH buffaloes suffered from a rectal temperature of 38.9^oc, slightly increased Heart rate, decreased body weight, anorexia, stiffness in gait, pale mucous membrane of vulva and conjunctiva, decreased milk production, ruminal hypo motile and constipation and Biochemical analysis showed that the buffaloes with hypophosphatemia had significantly lower average values of total protein (TP) and albumin in contrast to the apparently healthy group. They also had a significantly higher mean value in comparison to the control group in liver enzymes as aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Furthermore, the buffaloes with hypophosphatemia had significantly lower mean values of glucose 6 phosphate (G-6-P), calcium (Ca2+), phosphorus (P-), potassium (K+), and chloride (CL-) compared to the control group. This concludes that as a result of increased liver enzymes brought on by liver illnesses, phosphorus shortage results in defects in G-6-P and cellular energy metabolism of adenosine triphosphate (ATP). These defects cause ruminal hypo motility and ruminal stasis, which in turn cause economic losses owing to weight loss and a decrease in milk output.