For this investigation, 160 Gilthead seabream fish samples with external body surface hemorrhages, exophthalmia, and ocular opacity were obtained from the governorate of Portsaid, Egypt. Following thorough clinical, postmortem, and bacteriological assessments, samples were obtained from freshly dead fish. About 116 V. alginolyticus isolates were recovered from the examined fish after a bacteriological analysis. The examined fish showed many clinical signs such as hemorrhages on the external body surface, the base of fins, exophthalmia noticed, and the most common postmortem findings were pale, friable liver with hemorrhagic patches, bloody fluid, pale gills, and congested kidney The liver showed the greatest frequency of V. alginolyticus, followed by the kidney and Gills. On TCBS media, V. alginolyticus exhibited characteristic yellow colonies. The recovered isolates appeared as Gram-negative, non-spore-forming, curving rods or commas. All recovered isolates tested negative for urease and ONPG but positive for oxidase, catalase, lysine decarboxylase, ornithine decarboxylase, indole, and citrate utilization. Our research emphasizes the emergence of V. alginolyticus in seabream