Canine parvovirus-2 (CPV-2) is one of the most common causes of diarrhea in dogs. This study aimed to investigate the CPV-2 infection in diarrheic dogs in Ismailia governorate, Egypt, from September 2021 to July 2022. A total of 50 fecal samples were collected from bloody diarrheic dogs who visited veterinary clinics. Data including type of food, either eating outside or not, type of housing, contact with stray dogs or not, and vaccination history for each animal were collected. Fifty out of 50 (100%) were positive for CPV-2 using conventional PCR. We correlate between percentage of positive samples and some variables. In our study, more CPV-2 positive cases were reported in dogs eating cooked food in relation to type of food, not eating outside in relation to either eating outside or not, living with their owners within the same apartment in relation to type of housing, not contact with stray dogs in relation to either contact stray dogs or not, from non-vaccinated dogs and improperly vaccinated dogs in relation to vaccination status