Aerococcous spp. thought to be a harmless pathogen, it is now recognized as a promising disease-causing microorganism. In the field of microbiology, Aerococci is frequently confused with streptococci, leading to incorrect diagnoses. Antimicrobial resistance is globally increasing which is reflected in public health threats. Therefore, the current study was performed to evaluate the incidence of A. viridans in one hundred fecal swabs collected from broilers and layers farms in the Sharkia government, Egypt, and described its antibiotic resistance by the VITEK system. Only one A. viridans isolate was identified from 100 samples. A. viridans isolate was susceptible to vancomycin, Trimethoprim/ sulfamethoxazole, and linezolid, while, A. viridans isolate was resistant to Penicillin G, meropenem, tetracycline, and Ceftriaxone. As the first study in Egypt, this study concluded that MDR A. viridans is of importance in public health and plays an essential role in diarrhea infection in poultry, and is considered a hazard to human health.