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307787

Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Bacteriology, Immunology and Mycology

Abstract

Campylobacter spp < /em>. causes severe issues in chickens. In this investigation. Campylobacter species were isolated (182) in Dakahlia Governorate from 440 broilers and 440 ballade breeds.   Campylobacter coli 42 isolates(4%)and Campylobacter jejuni140isolates (16%). For C. jejuni, rates of recovery from various internal organs were 60%, 24%, 10%, and 5%, from the cloacal swab, heart, liver, and gizzard respectively. The morphological studies revealed that the majority of species from Campylobacter are motile, survive at 37-42Co, and are G-ve, slender spirally curled. They require 5%O2, 85%N2, and 10%CO2 to create a colony that looks like a dew drop on mCCDA media. By using API20, the suspected isolates were identical to typical Campylobacter

DOI

10.21608/scvmj.2023.307787

Keywords

Campylobacter jejuni, C. coli, Poultry, API 20E and PCR

Authors

First Name

Mahmoud

Last Name

El Sayed

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Faculty of Veterinary medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Abo Elkheir

Last Name

Esawy

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

Email

esawy55@gmail.com

City

-

Orcid

-

First Name

Mona

Last Name

Sobhey

MiddleName

-

Affiliation

Animal Reproduction Research institute.

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

Eidaroos

MiddleName

-

Affiliation

Bacteriology, Immunology and Mycology Department, Faculty of Veterinary medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Rania

Last Name

Khattab

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

Email

-

City

-

Orcid

-

First Name

Tamer

Last Name

ELfeky

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Abdelrahman

MiddleName

-

Affiliation

Animal Health Research Institute, Mansoura Lab. Agriculture Research Center (ARC)

Email

drraghdagabal85@gmail.com

City

-

Orcid

-

Volume

28

Article Issue

1

Related Issue

40091

Issue Date

2023-06-01

Receive Date

2023-02-10

Publish Date

2023-06-01

Page Start

157

Page End

166

Print ISSN

1110-6298

Online ISSN

2682-3284

Link

https://scvmj.journals.ekb.eg/article_307787.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=307787

Order

12

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Comparative Study Between Traditional Methods, Commercial Biochemical Test and PCR for Identification of Campylobacter Species Isolated from Poultry Farms

Details

Type

Article

Created At

24 Dec 2024