The present study was conducted to identify and isolate the bacterial pathogens that cause gastrointestinal problems in chickens, leading to a decrease in their production. The research also aimed to determine the incidence of these pathogens and their sensitivity to antibiotics. To achieve this, one hundred samples were collected from the cloaca of chickens suffering from diarrhea in four different private farms located in Bilbies Sharqiyah Governorate and were analyzed using bacteriological methods. The results revealed that there were 5 isolates: 39% E. coli, 22% C. perfringens,14% K. pneumoniae and 14% P. mirabilis, 11% P.vulgaris along with no detection of Salmonellae (0%). All 14 K. pneumoniae isolates previously identified using the traditional methods were identified rapidly and automatically by VITEK 2 system (bioMerieux, Craponne, France) using AST and ID-GNB cards, Vitek 2 software, Advanced Expert System [AES] software, and all 14 isolates were sensitive for 24 different antimicrobial agents: Cefazolin, Cefepime, Ciprofloxacin, Gentamycin, Tobramycin, Cefuroxime, Piperacillin, Amikacin, Meropenen, Cefotaxime, Trimethoprim sulfa, Ceftazidime, Levofloxacin, Chloramphenicol, Imipenem, Aztreonam, Cefoxitin, Amoxicillin/CA, Ticarcillin/CA, Minocycline, Ertapenem, Tigecycline, Moxifloxacin, Ampicillin/sulbactam.