In the present research work, 800 tissue samples were collected from the lungs, air sacs, liver, and spleen of 200 diseased broiler chickens (35-50 days old). These samples were used to isolate and identify Klebsiella species by traditional methods. The results showed that Klebsiella was recovered from 28 (3.5%) out of 800 examined samples and the isolates were differentiated into K. pneumoniae, 75 % (21/28), and K. oxytoca, 25 %(7/28). The rate of recovery of Klebsiellae was 6.5%, 3%, 2.5%, and 2% from the lungs, air sacs, liver, and spleen, respectively. The PCR assay was conducted to confirm the isolation results by detection of the common genes of Genus Klebsiella. The PCR assay was used to detect whether K. pneumoniae isolates have virulence and antimicrobial resistance genes or not. For that, five isolates that were strongly positive for biochemical tests were selected. All 5 (100%) isolates were found to have fimH and traT virulence genes and 4 (80%) isolates were found to have magA virulence gene. While all 5 (100%) isolates were found to have tetA(A) (Tetracycline resistance gene), blaTEM (ampicillin resistance gene), and mphA (erythromycin resistance gene) antibiotic resistance genes. Antimicrobial sensitivity testing for some K. pneumoniae isolates revealed that 20 out of 20 (100%) tested K. pneumoniae isolates, were resistant to ampicillin and oxytetracycline, 18 out of 20 (90%) tested isolates, was resistant to erythromycin, 16 out of 20 (80%) tested isolates, was resistant to streptomycin, 14 out of 20 (70%) tested isolates, was resistant to cefotaxime, 13 out of 20 (65%) tested isolates, was resistant to gentamycin,  9 out of 20 (45%) tested isolates, was resistant to neomycin and 6 out of 20 (30%) tested isolates, were resistant to chloramphenicol. While, the isolates were less resistant to ciprofloxacin and norfloxacin by a percentage of 20% and 10%, respectively.