In this study, 60 naturally infected seabreams (Sparus aurata) were randomly collected from several fish farms in Port-Said Governorate. Fish were subjected to clinical, postmortem, bacteriological, and molecular examinations. Furthermore, the organoleptic changes of fish fillets, including odor, color, general appearance, slime, and flesh consistency, were investigated. Grossly, the infected fish showed exophthalmia, cloudiness in the eyes, and abdominal ascites with a prolapsed vent. The visceral organs also appeared enlarged and varied in color from pale to congest the following dissection. Bacteriologically, Vibro parahaemolyticus (44.8%), Aeromans hydrophila (34.5%), and Pseudomonas aeruginosa (20.7%) were recovered from the diseased fish. Molecular studies of the retrieved isolates detected various virulence genes, such as trh for V. parahaemolyticus, aerolysin for A. hydrophila, toxA, and pslA for P. aeruginosa. Following 7 days of ice storage, the control samples were in acceptable condition, whereas the P. aeruginosa-inoculated samples became inedible and only usable after 4 days of storage. The above-mentioned findings showed the importance of implementation a strict control measures to combat the problems posed by resistant pathogens in the mariculture industry that cause food spoilage