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307333

Antimicrobial Resistance and Virulence Genes of Shiga Toxin-producing Escherichia coli (STEC) Isolated from Tilapia and Mullet and its Public Health Significance

Article

Last updated: 04 Jan 2025

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Tags

Zoonoses

Abstract

Shiga toxin-producing Escherichia coli (STEC) is responsible for several food-borne outbreaks worldwide. In this study, tissue samples of finfish (tilapia, n = 100) and (mullet, n = 100), and twenty human hand swabs from fish sellers and fishermen were tested bacteriologically for STEC presence. Isolates were tested for their antibiotic susceptibility and examined for the presence of the eaeA,stx1, and stx2 genes. E. coli and STEC were identified from the tissues (36.5% and 12.5%) of the examined tilapia and mullet, respectively; however, E. coli and STEC from human hand swabs were as high as (60% and 40%), respectively. Of the recovered E. coli isolates, 25 presumptive STEC (17 from finfish and 8 from humans) yielded characteristic mauve-colored colonies on the CHROMagar STEC medium. The highest prevalence of STEC was in mullet and tilapia from freshwater of Nile tributaries at 24% and 48%, followed by fish from freshwater fish farms at 16% and 12%, respectively. No STEC was isolated from fish from Suez Canal water and saltwater fish farms. Recovered STEC isolates from fish belonged to 14 serotypes belonging to (O121:H7, O113:H4, O119:H6, O128:H2, O153:H2, O91:H21, O26:H11, O44:H18, O146:H2, O55:H7, O124, O159, O78, and O117: H4). Isolates from human hand swabs belonged to (O26:H11, O91:H21, O15:H2, O121:H7, and O119:H6). One or two Shiga-toxin (stx1 and stx2) genes were confirmed in STEC isolates. STEC isolates from finfish and humans were multi-drug resistant (MDR). This study reported a high degree of contamination of freshwater finfish from the Ismailia governorate with STEC and highlighted the high level of antimicrobial resistance exhibited which is very hazardous to consumers.

DOI

10.21608/scvmj.2023.307333

Keywords

Fin fish, Zoonoses, E. coli, STEC, Virulence genes, Antimicrobial resistance

Authors

First Name

Dheyazan

Last Name

Al Qabili

MiddleName

M. Ali i

Affiliation

Agriculture and Veterinary Medicine College, Thamar University, Republic of Yemen Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

Email

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City

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Orcid

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First Name

Abdel‑Karim

Last Name

Aboueisha

MiddleName

M.

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Youssef

MiddleName

I.

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

El‑Mahallawy

MiddleName

S.

Affiliation

2Department of Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.

Email

-

City

-

Orcid

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Volume

28

Article Issue

1

Related Issue

40091

Issue Date

2023-06-01

Receive Date

2023-01-13

Publish Date

2023-06-01

Page Start

55

Page End

72

Print ISSN

1110-6298

Online ISSN

2682-3284

Article File

SCVMJ_Volume 28_Issue 1_Pages 55-72.pdf

PDF . 1.1MB

Link

https://scvmj.journals.ekb.eg/article_307333.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=307333

Order

5

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Antimicrobial Resistance and Virulence Genes of Shiga Toxin-producing Escherichia coli (STEC) Isolated from Tilapia and Mullet and its Public Health Significance

Details

Type

Article

Created At

24 Dec 2024