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289215

Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish

Article

Last updated: 24 Dec 2024

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Subjects

-

Tags

Bacteriology, Immunology and Mycology

Abstract

A total of, (100) samples of O. Niloticus and (100) samples of C. Gariepinus were gathered from multiple areas in Dakahlia governorate from April 2019 to April 20. Fish samples were subjected to clinical and microbiological examination from the kidney, liver, and spleen. Isolates were characterized by cultural characters, some biochemical tests, Api 20 system, and PCR. 24 diseased fish were characterized as infected with Y. ruckeri [10 from 100] O. niloticus (10%) and 14 from C. Gariepinus (14%)] with 44 Yersinia ruckeri isolates with the percentage of 22%. 16srRNA gene (specific common gene) was demonstrated in all Y.ruckeri isolates by PCR in addition to the detection of virulence genes (yrp,yrIlm,yhlA,yhlB,yrInv) in them by PCR assay and multi-drug resistance genes (blaTEM,qnrS, tetAgene). The study results showed that PCR is a rapid and reliable method for Y. ruckeri isolates identification which will be useful in the prevention and control of Yersiniosis.

DOI

10.21608/scvmj.2023.289215

Keywords

Yersinia ruckeri, Oreochromis niloticus, Clarias gariepinus, MDR and PCR

Authors

First Name

Ahmed

Last Name

Khafagy

MiddleName

-

Affiliation

1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

Email

dr.ahmedkhafagy@gmail.com

City

-

Orcid

-

First Name

Abo Elkheir

Last Name

Esawy

MiddleName

-

Affiliation

Department of Bacteriology, Animal Health Research Institute (AHRI), Mansoura lab. Agriculture Research Center (ARC).

Email

esawy55@gmail.com

City

-

Orcid

-

First Name

Ali

Last Name

Wahdan

MiddleName

-

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

Email

-

City

-

Orcid

-

First Name

Tamer

Last Name

El feky

MiddleName

-

Affiliation

Department of Bacteriology, Animal Health Research Institute (AHRI), Mansoura lab. Agriculture Research Center (ARC).

Email

-

City

-

Orcid

-

First Name

Sohila

Last Name

El-Deeb

MiddleName

-

Affiliation

Free veterinarian

Email

-

City

-

Orcid

-

First Name

Marwa

Last Name

Abo Hashem

MiddleName

-

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University

Email

marwashassan@vet.suez.edu.eg

City

-

Orcid

-

Volume

28

Article Issue

1

Related Issue

40091

Issue Date

2023-06-01

Receive Date

2022-11-18

Publish Date

2023-06-01

Page Start

1

Page End

15

Print ISSN

1110-6298

Online ISSN

2682-3284

Article File

SCVMJ_Volume 28_Issue 1_Pages 1-15.pdf

PDF . 995.6kB

Link

https://scvmj.journals.ekb.eg/article_289215.html

Detail API

https://scvmj.journals.ekb.eg/service?article_code=289215

Order

1

Type

Original Article

Type Code

992

Publication Type

Journal

Publication Title

Suez Canal Veterinary Medical Journal. SCVMJ

Publication Link

https://scvmj.journals.ekb.eg/

MainTitle

Molecular Characterization and Multidrug Resistance of Yersinia Species in Fish

Details

Type

Article

Created At

24 Dec 2024