Coded Thirty actinobacteria isolates were isolated from the rhizosphere of different plants in the secluded desert to select the promising isolate that has a wide antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. The most active isolate was coded as AMM1, and it was characterized physically, biochemically, and genetically as Streptomyces sp., and showed 99% similarity with Streptomyces geysiriensis when compared with the 16S sequences deposited in GenBank. The temperature was 35 °C, the pH was 7, and the best timing was 12 days as the best environmental condition suitable for growth after studying. The agar-well diffusion method was used after the metabolites were mixed with several polar organic solvents (Hexane, chloroform, ethyl acetate, and ethanol) to determine the most effective antibacterial crude extract, and the results found that ethyl acetate crude extract was the most effective one among them, and their minimum inhibition concentration was 2.5 µg/ml recorded against E. coli ATCC7839 with a value of 9.0 mm, Candida albicans ATCC10321 with a value of 9.33 mm and 8.33 mm against Bacillus subtilis ATCC6633. The same solvent extract produced a mean inhibition zone of 8.33 mm against P. aeruginosa ATCC9027 and 9.33 mm against S. aureus ATCC6538 at a minimal inhibitory concentration of 5 µg /ml.