Two weekly visits to the ocular microbiology lab, Ophthalmology Hospital, Tanta University, were conducted to collect patient data and fungal samples from clinically diagnosed mycotic keratitis cases. It was observable that fungal corneal infections were more common during summer season (48 cases), then spring (26 cases), 2022. This type was more abundant among male patients of age ranged between 20 - 50 years (43 cases out of 100 total mycotic ulcers); in habiting rural houses (78 cases); represented mostly among students and indoor workers (45 cases), that were closely related to commercial contact lenses usage (37 cases). Lab investigation showed more frequency of Aspergillus flavus (58 cases), followed by Aspergillus niger (22 cases) among the isolated 100 mycotic ulcers. Eight Egyptian wild plants were collected from Halayeb and Shalateen region; their extracts were tested for antifungal activity, whereas the most effective plant extracts against A. flavus recorded MIC values of 12.5 mg/ml for Senna occidentalis, followed by 25 mg/ml for Pulicaria undulata, then 50 mg/ml for Aerva Jovanica. The highest antifungal effect of Senna occidentalis as illustrated by TEM, showing complete rupture of cell wall, cell membrane, elongation, deformation of cell shape, cytoplasmic agglutination, disintegration of nuclear membrane, shrinking of cytoplasm and leakage of intracellular component outside the damage cell boundaries.