Football sport is a public sport that is widely distributed around the world. Football players are subject to the same skin conditions as others, but participation in a sports environment characterized by unique stresses on the skin that are not normally seen in inactive individuals can cause skin issues. Nano-technology approach involving the synergistic interaction between microorganisms and nanoparticles offers an affordable, environmentally, and beneficial solution for fungal skin infection prevention. Herein, CeO2NPs were properly characterized using different spectral analyses. Moreover, different fungal species were systematically isolated from the eleven volunteer football players' different skin parts, Port Said sporting city, Port Said, Egypt, and grown on a modified sabouraud dextrose agar medium. Eleven pure-culture fungal isolates were identified using spacer between 18S and 28S rRNA genes identification technique. The growth-inhibiting effect of different CeO2NPs concentrations on the fungal isolates after ten days of incubation was studied. Furthermore, the reduction activity of used CeO2NPs towards the isolated fungal species was inclusivity researched. The outcome data experimentally symbolized that the fungal isolates were remarkably stable under a pH of 5.5 and temperature of 25°C (optimized conditions). Besides, the growth-inhibiting of different fungal isolates was directly proportional to increasing the concentration of CeO2NPs and completely stopped at the optimum dosage of 12 g/L compared to the control sample (non-treated CeO2NPs specimen). Overall, the present study illustrates an ample perspective of cerium oxide nanoparticles as a futuristic platform for the decontamination of fungal skin diseases.